Introduction: Psammoma bodies (PBs) are calcined structures with a concentric lamellar structure. These laminar bodies are characteristic of low- and highgrade serous papillary ovarian carcinomas with an incidence of 100% and 50%, respectively. They begin to develop in the early stages of carcinogenesis, which is important in the early and / or differential diagnosis of ovarian tumors. 

Aim: The work is to carry out morphometrical analysis of the tissues serous ovarian carcinoma with psammoma bodies. 

Materials and methods: We have analyzed the samples from 30 serous ovarian carcinoma patients by using histology and histochemistry. All photos were captured with the digital visualization system based on Zeiss Primo Star microscope with digital camera ZEISS Axiocam ERC 5s and software package “Zen 2.0” (Carl Zeiss, Germany). Classification of the size of psammoma bodies was carried out using the method of ROC analysis. The graphical representation of statistical analysis results was performed using the GraphPad Prism 7.04.

Results: The most common of PBs were fibrous-based tumor papillae of serous ovarian carcinoma and tumor detritus. A significant amount of PBs was in the thickness of the connective tissue of the tumor nodes, as well as the adjacent intact tissues of the ovaries. In some cases, PBs was surrounded by remnants of vascular capillary walls. PBs had a layered structure, often represented by fragments and debris that stored the primary structure of the biomineral object. The amount of PBs in tumors and ovarian tissue ranged from 1 to 200 units. In our study, the size of the PBs of serous ovarian carcinoma ranged from 12.62 to 493.67 μm. Using ROC analysis and construction of a heat map, we investigated the distribution of PBs in the studied group of samples of serous ovarian carcinoma. In general, PBs by size characteristics can be divided into 3 groups: large – more than 200 μm (visible on ultrasound), 71 – 199 μm – medium, have satellites and a tendency to merge, and small – less than 70 μm.

Conclusions: PBs is an important diagnostic feature of the cancer ovary, which can be used in ultrasound and histological methods. PBs can be divided into 3 groups by size – large, medium, and small.