Introduction: Adhesive properties are an important feature of biomaterial surface in implant osseointegration. Following parameters can influence to this process: morphology, topography, roughness, chemical composition, surface energy and composition, chemical potential, residual stress, impurities, and the presence of metallic and nonmetallic compounds. The cause of adhesion is the molecular attraction of the contacting phases or their chemical interaction and to control surface properties we can increase the success adhesion rate of implants. There are several methods for adhesion properties as peel tests, scratch tests and blister tests. However, these measurements do not allow to characterize surface behavior at the physiological conditions.  Microfluidic devices are one of the in-vitro biosystems. Nevertheless, it is widely used for cancer detection, tumor cell isolation, and screening of therapeutics.

Aim: To develop a simple and effective dynamic test with several containers in a simulated physiological environment under constant flow rate and medium exchange.

Materials and methods: The construction of the dynamic fluid circulation system consisted of connected containers by the medical silicone tubes, and a peristaltic pump mimics the liquid flow. The dynamic flow conditions were chosen according to the physiological model to test the pure Mg samples and with silicate-based coating. The dynamic system were filled with 10⁵ CFU/mL S.aureus suspension in nutrient broth at 37°C. The adhesive properties were defined via surface printing on solid agar after the cultivation of 2, 4, and 6 h, followed by the streak plate technique.

Results: The bacterial adhesion was quantitatively determined by counting the number of colonies. Colonies were not found in the Mg groups at all timelines. In comparison, there were small colonies of 10² CFU/mL on the Mg samples with oxide coating at 2 and 4 hours. And it has increased slightly to 10³ CFU/mL after 6 hours.

Conclusions: Developed setup demonstrated a promising in vitro method to investigate adhesion properties at in-vivo conditions by maintaining physiological circulation. This is particularly important concerning the examination antibacterial capacity of the surfaces.

Acknowledgment: This research was funded by EU-H2020-MSCA-RISE (grant no 777926), a grant from Ministry of Education and Science of Ukraine (0120U101972, 0119U100770) and Ukraine National Research Fund (2020.02/0223).