Diedkova K.*, Roshchupkin A.*, Yevheniia Husak*,**, Sergiy Kyrylenko*
*Sumy State University, Sumy, Ukraine
**Silesian University of Technology, Gliwice, Poland
Toufik’s Medical Journal
Volume 1, Supplement 1, November 2021
Abstract from Biomedical Perspectives III
Introduction: 3D Ti6Al4V implants with triply periodic minimal surface (TPMS) structures are new generation in the medical industry. Due to their biomimetic architecture, porous titanium materials exhibits a great potential in medical fields as orthopedic implants. Porous scaffolds could mimic natural substrates by the regulation of pore geometry and surface area that will suitable for cell attachment, migration, and proliferation. But the issue of improving surface osteocompatibility remains open. The osteocompatibility of implants can be enhanced by surface bioactivation. One of the simple and cheap surfaces modification methods is alkaline treatment. Obtained titanate coating enhances surface morphology, increases wettability and mineralization process based on our previous study.
Aim: The aim of our research was to evaluate the bioactivity of Ti-3D TPMS scaffolds with alkali-based surface modifications.
Materials and methods: All implant surfaces were received alkali-based treatment or were left untreated (controls). The sterilized scaffolds were placed in a separate well of a 24-well cell culture plate. Human umbilical cord mesenchymal stem cells (UCMSC) were plated at a density of 20 000 cells/cm 2. A resazurin reduction assay evaluated cell viability at 1st, 3d and 5th day of cultivation. The fluorescence microscopy assay confirmed cells adhesion.
Results: Resazurin reduction assay did not show notable differences in the cell viability in the control and experimental group at first-day cultivation. However, the proliferation rate of the UCMSC cells was higher than the control wells after 5 days of cultivation. DAPI staining revealed cell adhesion to the scaffold surfaces in both control and experimental groups.
Conclusions: The alkali-modified surface demonstrate biocompatibility and bioactivity based on cell proliferation data. The submicron-structured alkali-titanate layer is a promising treatment for Ti-based surface modification.
Acknowledgment: This research was funded by Ministry of Education and Science of Ukraine 0119U100823.