Introduction: Monitoring of antibiotic resistance of isolates from wounds allows to assess the future prospects for antibiotic therapy and the current situation in the hospitals. At the same time, the search for promising antibiotics and antimicrobial substances for new treatment strategies and overcoming antibiotic resistance of microorganisms is continued.

Aim: The aim of the study was to detect bacteria associated with wound surfaces of patients from Kyiv Regional Clinical Hospital (Kyiv) for the period of April 2019 – April 2020, and check their resistance to the most commonly used antibiotics. 

Materials and methods: Isolation, identification and antibiotic resistance estimation were performed according to generally accepted methods and in accordance with international recommendations of EUCAST. 

Results: In total, 1540 isolates were analyzed. The most part of isolates from wound surfaces were presented by Staphylococcus epidermidis and S. aureus. Among the gram-negative microflora Enterobacteriaceae was dominated and presented by Klebsiella pneumoniae, Escherichia coli and others. The isolates  were mostly resistant to fosfomycins, penicillins, β-lactam inhibitors and monobactams. At the same time, they were highly sensitie to the last line antibiotics – teicoplanin, vancomycin and linezolid.

Conclusions: The obtained data are important according to the assessment of antibiotic resistance development in hospitals in Ukraine and the prospects of developing new strategies for the treatment of antibiotic-resistant infections.